Dr. rer. nat. Kerstin Seyfarth

Methods

 

Microbiology

cultivation and isolation of aerobic and anaerobic microorganisms

Practical work with geneticaly modified strains, protective group SI and SII)

Practical work with human-pathogenic germs, risk group R2

microbiological characterisation of microorganisms

Phase contrast microscopy

Fluorescence microscopy

Analysis of the nutrient behaviour

 

Molekular biology

Genomics

PCR

DNA-Gelekrophoresis

Denaturated and temperature gradienten gel elektrophoresis (DGGE/TGGE)

side directed mutagenesis

Restriktion-lenght-polymorphism (RFLP)

Specific amplified DNA PCR (SAPD-PCR)

DNA-sequenzing (Sanger)

Proteomics

Heterologous gene expression in E. coli

Purification

Dialysis

SDS gel elektrophoresis

Activity tests

Hydrolases (Proteases, Amylases Lipases, Cellulases)

Sulfur Oxygenase-/ Reductase from Acidianus ambivalens

 

Chemical analysis

Gas chromatography

Fast liquid protein chromatography (FPLC)

High performance liquid chromatography (HPLC)

Volatile acids

Amino acids

Sugar

Fourier transformation infrared spectrometry (FTIR)

UV-Vis spectrometry

 

Software 

MS Windows Office

Mac Office

Software for chemicaly analytical systems

Fast liquid protein chromatography (FPLC, Pharmazia)

High performance piquid chromatography (HPLC, LC-Solution, Shimadzu)

Gas chromatography (GC, Lab Solution GC, Shimadzu)

Fourier transformation infrared spectrometry (FTIR, Shimadzu)

UV-Vis spectrometry (Beckmann)

Phylogenetische-Software

ARB

TreeView 1.6.6

Clustal 2.1

Phylip 3.65

MEGA 4.1

DNASIS-Mac V2.0

für Linux Sun

Software for the computer assisted molecular biological identifaction system MicroSeq(R)

Fluorescence microskopy softwares (Zeiss, Keyance)

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